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the corresponding zetaview software (8.03.04.01)  (Particle Metrix)
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Inhibition of EV secretion by PDDC and 5 in glial cells. Primary astrocytes were treated in parallel incubations with PDDC, 5, or cambinol in the 0.03–30 μM range as indicated; DMSO (0.02%) was used as vehicle control (set as 100% on y‐axis). Media was collected after 2‐hr incubation and centrifuged at 2,700 g for 15 min at 4°C. Supernatant was collected and the number of extracellular vesicles (EVs) was quantified using <t>ZetaView</t> Nanoparticle Tracker. The data points correspond to per cent inhibition of EV release (±SEM) over a concentration range for each compound; 100% release corresponds to 4.87 × 107 ± 0.098 EVs. Results are the average of three or four independent assays. Each independent assay was the average of three replicates. pEC50 values and corresponding 95% confidence asymmetrical intervals, CI (95%), were obtained from a non‐linear fit of log [inhibitor] against response, using GraphPad Prism 7
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Figure 7. LBDXL Hepatocytes Could Repopu- late Fah/ Mouse Livers (A) Kaplan-Meier curve showed the survival rate of cell-transplanted Fah/ mice after removing the supply of NTBC. (B and C) After immunohistochemical staining of Fah, liver repopulation by LBDXL hepatocytes and FH was quantified using <t>ImageJ</t> software (B). Immunohistochemical staining of Fah was shown in (C). Data are expressed as means ± SEMs. (D) The transplanted cells demonstrated mature hepatocyte phenotypes with the expression of Fah, Alb, and the bile canaliculus marker Dpp4. The scale bar represents 200 mM. See also Figure S7.
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Figure 7. LBDXL Hepatocytes Could Repopu- late Fah/ Mouse Livers (A) Kaplan-Meier curve showed the survival rate of cell-transplanted Fah/ mice after removing the supply of NTBC. (B and C) After immunohistochemical staining of Fah, liver repopulation by LBDXL hepatocytes and FH was quantified using <t>ImageJ</t> software (B). Immunohistochemical staining of Fah was shown in (C). Data are expressed as means ± SEMs. (D) The transplanted cells demonstrated mature hepatocyte phenotypes with the expression of Fah, Alb, and the bile canaliculus marker Dpp4. The scale bar represents 200 mM. See also Figure S7.
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Figure 7. LBDXL Hepatocytes Could Repopu- late Fah/ Mouse Livers (A) Kaplan-Meier curve showed the survival rate of cell-transplanted Fah/ mice after removing the supply of NTBC. (B and C) After immunohistochemical staining of Fah, liver repopulation by LBDXL hepatocytes and FH was quantified using <t>ImageJ</t> software (B). Immunohistochemical staining of Fah was shown in (C). Data are expressed as means ± SEMs. (D) The transplanted cells demonstrated mature hepatocyte phenotypes with the expression of Fah, Alb, and the bile canaliculus marker Dpp4. The scale bar represents 200 mM. See also Figure S7.
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Figure 7. LBDXL Hepatocytes Could Repopu- late Fah/ Mouse Livers (A) Kaplan-Meier curve showed the survival rate of cell-transplanted Fah/ mice after removing the supply of NTBC. (B and C) After immunohistochemical staining of Fah, liver repopulation by LBDXL hepatocytes and FH was quantified using <t>ImageJ</t> software (B). Immunohistochemical staining of Fah was shown in (C). Data are expressed as means ± SEMs. (D) The transplanted cells demonstrated mature hepatocyte phenotypes with the expression of Fah, Alb, and the bile canaliculus marker Dpp4. The scale bar represents 200 mM. See also Figure S7.
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Figure 7. LBDXL Hepatocytes Could Repopu- late Fah/ Mouse Livers (A) Kaplan-Meier curve showed the survival rate of cell-transplanted Fah/ mice after removing the supply of NTBC. (B and C) After immunohistochemical staining of Fah, liver repopulation by LBDXL hepatocytes and FH was quantified using <t>ImageJ</t> software (B). Immunohistochemical staining of Fah was shown in (C). Data are expressed as means ± SEMs. (D) The transplanted cells demonstrated mature hepatocyte phenotypes with the expression of Fah, Alb, and the bile canaliculus marker Dpp4. The scale bar represents 200 mM. See also Figure S7.
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graphpad prism version 6.04  (USACO Corporation)
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Figure 7. LBDXL Hepatocytes Could Repopu- late Fah/ Mouse Livers (A) Kaplan-Meier curve showed the survival rate of cell-transplanted Fah/ mice after removing the supply of NTBC. (B and C) After immunohistochemical staining of Fah, liver repopulation by LBDXL hepatocytes and FH was quantified using <t>ImageJ</t> software (B). Immunohistochemical staining of Fah was shown in (C). Data are expressed as means ± SEMs. (D) The transplanted cells demonstrated mature hepatocyte phenotypes with the expression of Fah, Alb, and the bile canaliculus marker Dpp4. The scale bar represents 200 mM. See also Figure S7.
Graphpad Prism Version 6.04, supplied by USACO Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 7. LBDXL Hepatocytes Could Repopu- late Fah/ Mouse Livers (A) Kaplan-Meier curve showed the survival rate of cell-transplanted Fah/ mice after removing the supply of NTBC. (B and C) After immunohistochemical staining of Fah, liver repopulation by LBDXL hepatocytes and FH was quantified using <t>ImageJ</t> software (B). Immunohistochemical staining of Fah was shown in (C). Data are expressed as means ± SEMs. (D) The transplanted cells demonstrated mature hepatocyte phenotypes with the expression of Fah, Alb, and the bile canaliculus marker Dpp4. The scale bar represents 200 mM. See also Figure S7.
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Figure 7. LBDXL Hepatocytes Could Repopu- late Fah/ Mouse Livers (A) Kaplan-Meier curve showed the survival rate of cell-transplanted Fah/ mice after removing the supply of NTBC. (B and C) After immunohistochemical staining of Fah, liver repopulation by LBDXL hepatocytes and FH was quantified using <t>ImageJ</t> software (B). Immunohistochemical staining of Fah was shown in (C). Data are expressed as means ± SEMs. (D) The transplanted cells demonstrated mature hepatocyte phenotypes with the expression of Fah, Alb, and the bile canaliculus marker Dpp4. The scale bar represents 200 mM. See also Figure S7.
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Image Search Results


Inhibition of EV secretion by PDDC and 5 in glial cells. Primary astrocytes were treated in parallel incubations with PDDC, 5, or cambinol in the 0.03–30 μM range as indicated; DMSO (0.02%) was used as vehicle control (set as 100% on y‐axis). Media was collected after 2‐hr incubation and centrifuged at 2,700 g for 15 min at 4°C. Supernatant was collected and the number of extracellular vesicles (EVs) was quantified using ZetaView Nanoparticle Tracker. The data points correspond to per cent inhibition of EV release (±SEM) over a concentration range for each compound; 100% release corresponds to 4.87 × 107 ± 0.098 EVs. Results are the average of three or four independent assays. Each independent assay was the average of three replicates. pEC50 values and corresponding 95% confidence asymmetrical intervals, CI (95%), were obtained from a non‐linear fit of log [inhibitor] against response, using GraphPad Prism 7

Journal: British Journal of Pharmacology

Article Title: A novel and potent brain penetrant inhibitor of extracellular vesicle release

doi: 10.1111/bph.14789

Figure Lengend Snippet: Inhibition of EV secretion by PDDC and 5 in glial cells. Primary astrocytes were treated in parallel incubations with PDDC, 5, or cambinol in the 0.03–30 μM range as indicated; DMSO (0.02%) was used as vehicle control (set as 100% on y‐axis). Media was collected after 2‐hr incubation and centrifuged at 2,700 g for 15 min at 4°C. Supernatant was collected and the number of extracellular vesicles (EVs) was quantified using ZetaView Nanoparticle Tracker. The data points correspond to per cent inhibition of EV release (±SEM) over a concentration range for each compound; 100% release corresponds to 4.87 × 107 ± 0.098 EVs. Results are the average of three or four independent assays. Each independent assay was the average of three replicates. pEC50 values and corresponding 95% confidence asymmetrical intervals, CI (95%), were obtained from a non‐linear fit of log [inhibitor] against response, using GraphPad Prism 7

Article Snippet: Extra cellular vesicles were quantified using ZetaView Nanoparticle tracker (Particle Metrix GmBH, Meerbusch, Germany) and the corresponding Zetaview software (8.03.04.01). pEC 50 values and corresponding 95% confidence asymmetrical intervals, CI (95%), were obtained from a non‐linear fit of log [inhibitor] vs. response using GraphPad Prism 7.

Techniques: Inhibition, Incubation, Concentration Assay

Figure 7. LBDXL Hepatocytes Could Repopu- late Fah/ Mouse Livers (A) Kaplan-Meier curve showed the survival rate of cell-transplanted Fah/ mice after removing the supply of NTBC. (B and C) After immunohistochemical staining of Fah, liver repopulation by LBDXL hepatocytes and FH was quantified using ImageJ software (B). Immunohistochemical staining of Fah was shown in (C). Data are expressed as means ± SEMs. (D) The transplanted cells demonstrated mature hepatocyte phenotypes with the expression of Fah, Alb, and the bile canaliculus marker Dpp4. The scale bar represents 200 mM. See also Figure S7.

Journal: Cell reports

Article Title: Maintenance of Primary Hepatocyte Functions In Vitro by Inhibiting Mechanical Tension-Induced YAP Activation.

doi: 10.1016/j.celrep.2019.10.128

Figure Lengend Snippet: Figure 7. LBDXL Hepatocytes Could Repopu- late Fah/ Mouse Livers (A) Kaplan-Meier curve showed the survival rate of cell-transplanted Fah/ mice after removing the supply of NTBC. (B and C) After immunohistochemical staining of Fah, liver repopulation by LBDXL hepatocytes and FH was quantified using ImageJ software (B). Immunohistochemical staining of Fah was shown in (C). Data are expressed as means ± SEMs. (D) The transplanted cells demonstrated mature hepatocyte phenotypes with the expression of Fah, Alb, and the bile canaliculus marker Dpp4. The scale bar represents 200 mM. See also Figure S7.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Alb, goat polyclonal antibody Abcam Cat# ab19194; RRID:AB_777886 Anti-Hnf4a, rabbit polyclonal antibody Santa Cruz Biotechnology Cat# sc-8987; RRID:AB_2116913 Anti-Yap, mouse monoclonal antibody (Clone 63.7) Santa Cruz Biotechnology Cat# sc-101199; RRID:AB_1131430 Anti-Yap, mouse monoclonal antibody (Clone H-9) Santa Cruz Biotechnology Cat# sc-271134; RRID:AB_10612397 Anti-Fah, rabbit polyclonal antibody Abbomax Cat# 602-910 Anti-b-Catenin, mouse monoclonal antibody (Clone 14/Beta-Catenin) BD Biosciences Cat# 610154; RRID:AB_397555 Anti-E-Cadherin, rat monoclonal antibody (Clone DECMA-1) Santa Cruz Biotechnology Cat# sc-59778; RRID:AB_781738 Anti-Dpp4, goat polyclonal antibody R&D Systems Cat# AF954; RRID:AB_355739 Anti-Flag, mouse monoclonal antibody (Clone FG4R) Invitrogen Cat# MA1-91878; RRID:AB_1957945 Bacterial and Virus Strains AAV2/8-CAG-EGFP-T2A-Cre Obio Technology N/A AAV2/8-CAG-GFP Obio Technology N/A AAV2/8-EF1A-EGFP-P2A-Flag-Yap 5SA Obio Technology N/A AAV2/8-EF1A-EGFP-P2A-MSC-3Flag Obio Technology N/A Chemicals, Peptides, and Recombinant Proteins Chemical library See Table S1 Dexamethasone Sigma Cat# D4902 L-Ascorbic acid 2-phosphate Sigma Cat# A8960 N-2 Supplement (100X) Thermo Fisher Scientific Cat# 17502048 B-27 Supplement (50X), serum free Thermo Fisher Scientific Cat# 17504044 Matrigel, growth factor reduced Corning Cat# 356231 Dispase Thermo Fisher Scientific Catalog# 17105041 Collagenase, Type IV Thermo Fisher Scientific Catalog# 17104019 Critical Commercial Assays RNA sequencing Shanghai OE Biotech N/A Deposited Data Raw and analyzed data This paper GEO: GSE138158 Experimental Models: Organisms/Strains C57BL/6 and 129 mice Shanghai SLAC Laboratory N/A C57BL/6 Yapf/f mice Zhang et al., 2010 N/A Rosa26loxP-mTom-stop-loxP–mGFP (mTmG) mice Jackson Laboratory Cat# 007576 129 FAH / mice Overturf et al., 1997 N/A Oligonucleotides Primers for real-time PCR, see Table S2 This paper N/A Recombinant DNA pCMV-flag Yap2 5SA Zhao et al., 2007 Addgene Plasmid #27371 pAAV-EF1A-EGFP-P2A-MSC-3Flag Obio Technology N/A pAAV-EF1A-EGFP-P2A-Flag-Yap 5SA This study N/A Software and Algorithms NIS-Elements software Nikon N/A ImageJ NIH https://imagej.nih.gov/ij/download.html GraphPad Prism 7.04 GraphPad N/A R R Studio https://rstudio.com/products/rstudio/ Cell Reports 29, 3212–3222.e1–e4, December 3, 2019 e1

Techniques: Immunohistochemical staining, Staining, Software, Expressing, Marker